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. 2020 Jan 15;10:308. doi: 10.1038/s41598-019-57093-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Difference in the rate of decrease in pH in culture media determines the rate of viability loss of V. cholerae strains. pH changes in cultures of isogenic strains that contain functional VC1589 or non-functional VC1589 in the presence of 1% glucose were compared. (a) N16961 (circle) and DHL001 (filled circle), (b) DHL003 (circle) and B33 (filled circle), (c) DHL006 (circle) and IB4122 (filled circle), and (d) DHL007 (circle) and IB5230 (filled circle).