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. 2020 Jan 15;10:308. doi: 10.1038/s41598-019-57093-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of critical pH for loss of viability of V. cholerae. Approximately 10⁸ cells of a Wave 1 strain, N16961, and a Wave 3 strain, IB5230, grown in LB were harvested and resuspended in 0.1 M citrate buffer solution, the pH of which was adjusted to 5.1 (filled circle), 5.2 (circle), 5.3 (inverted filled triangle), 5.4 (inverted triangle), and 5.5 (filled square). Viable cells were counted on LB plates for up to 24 hours. Data were obtained from three independent experiments and a representative result of IB5230 is shown.