Skip to main content
. 2020 Jan 9;10:1538. doi: 10.3389/fphar.2019.01538

Figure 2.

Figure 2

NSA suppresses MLKL activation but not p-RIP3. Prevention of MLKL activation by treatment with 3 μM NSA for 24 h after OGD treatment. (A) NeuN and p-MLKL double staining. Cell nuclei were stained with DAPI (blue fluorescence) and neurons were stained with NeuN (red fluorescence). Pictures were taken using a fluorescence microscope (scale bar = 20 μm). (B) WB analysis of the level of p-RIP3 and p-MLKL induced by OGD, p-MLKL level was significantly reversed after NSA treatment. Data are presented as the mean ± SEM, n = 4. *p < 0.05, vs. the control group (CTL). # p < 0.05, vs. the OGD group. Data were analyzed using Student’s t-test. RIP3, receptor interacting protein kinase-3; OGD, oxygen-glucose deprivation; NSA, necrosulfonamide; MLKL, mixed-lineage kinase domain-like protein; Nec-1, necrostatin-1; WB, Western blot.