Figure 5.

The therapeutic window of NSA after SCI. The time of administration of NSA was 15 min, 30 min, 1 h, 3 h, 6 h, 12 h, and 24 h after surgery in groups, respectively. The behavioral performances conducted by forelimb grip strength test (A) and BMS score system (B) were observed at 1, 3, 7, 14, 21, and 28 days following SCI surgery. (C) Measurement of spinal edema at 3 days post-SCI. Data are reported as the mean ± SEM, n = 9. *p <0.05, vs. the SCI+Vehicle group. ^# p < 0.05, vs. the 24 h group. (A and B): Data were analyzed by two-way ANOVA followed by Tukey’s multiple comparison test. C: Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. SCI, spinal cord injury; NSA, necrosulfonamide; BMS, basso mouse scale.