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. 2020 Jan 15;10:409. doi: 10.1038/s41598-019-57295-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of indel locations. All the sequence variants were extracted from the group called “Perfect FLAG + indels”. The sequences were aligned to the reference gene containing the inserted sequence. The read count for each indel-containing variant was then converted to percentage of the total read count of variants from the “Perfect FLAG + indels” for all individuals. The percentages were plotted on the reference sequence as colored dots (a) AS 24 ODN (green), S 24 ODN (blue) and ds 24 ODN (red). (b) S 48 ODN (pink) and S 84 ODN (black). In order to analyze the difference in indel positions between the different groups, the percentages of indels located either at the 5′- or 3′-end of the inserted sequence was calculated for each group and is shown in (c).