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. 2020 Jan 15;10:390. doi: 10.1038/s41598-019-57319-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Engineering of Synechococcus elongatus UTEX 2973 for production of sucrose. (A) Metabolic pathways of carbohydrate biosynthesis in Synechococcus 2973. The sucrose permease CscB was expressed for sucrose excretion. Genes highlighted in blue encode enzymes for glycogen and sucrose production. In Synechococcus 2973, the SPS enzyme has both sucrose-phosphate synthase and sucrose-phosphate phosphatase activities. (B) Introduction of the cscB gene into the neutral site 3 (NS3). The primers used for PCR genotyping are highlighted in green. (C) PCR genotyping to confirm complete segregation of cscB. The wild type (WT) Synechococcus 2973 was used as a control. The PCR product of the WT gene is 514 base pairs (bp). No WT copy was present in the cscB-expressing strain. ADP-Glu, adenosine diphosphate glucose; CBB cycle, Calvin-Benson-Bassham cycle; CscB, sucrose permease; F6P, fructose 6-phosphate; G1P, glucose 1-phosphate; G6P, glucose 6-phosphate; S6P, sucrose 6-phosphate; UDP-Glu, uridine diphosphate glucose; glgA, glycogen synthase; glgC, ADP-glucose pyrophosphorylase; spp, sucrose-phosphate phosphatase; sps, sucrose-phosphate synthase.