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. 2020 Jan 15;10:390. doi: 10.1038/s41598-019-57319-5

Figure 3.

Figure 3

Analysis of carbohydrate metabolism in Synechococcus 2973 cscB-expressing strain. Cells were grown in BG11 medium with or without addition of 150 mM NaCl. (A) Biomass accumulation. (B) Sucrose production. (C) Glycogen content. (D) Semi-quantitative RT-PCR for monitoring gene expression in glycogen and sucrose syntheses. The housekeeping gene rpoA was used as a control. The numbers below the gel represent the relative band intensities from samples growing with 150 mM NaCl versus 0 mM NaCl. The full-length gels are presented in Supplementary Fig. S2. The data in Panels A–C represents mean ± sd from three biological replicates. DW, cell dry weight; rpoA, RNA polymerase subunit α.