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. 2020 Jan 15;10:390. doi: 10.1038/s41598-019-57319-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Engineering of the sucrose biosynthetic pathway in Synechococcus 2973. (A) Schematics of expressing the Synechocystis 6803 sps and spp genes in the 2973-cscB strain. (B) Sucrose production in sps and sps-spp expression strains. Cells were cultured in BG11 medium for 3 days without addition of NaCl. The “+” symbol indicates that the strain contains the indicated overexpressed enzyme(s). The data represents mean ± sd of three biological replicates. RBS, ribosome binding site.