Table 1.
Plasmids and strains used in this study.
| Name | Description | Reference |
|---|---|---|
| Plasmids | ||
| pRL443 | conjugal plasmid for bacterial conjugation | 29 |
| pRL623 | helper plasmid for bacterial conjugation | 29 |
| pSL2985 | PlacUV5-cscB (E. coli), lacI, NS3-targeting [CmR] | 13 |
| pSL3005 | PlacUV5-cscB (E. coli), lacI, NS3-targeting [KanR] | This study |
| pSL3037 | PlacUV5-cscB (E. coli), lacI, bom site, NS3-targeting [KanR] | This study |
| pSL3360 | Ptrc1O-sps (Synechocystis 6803), RSF1010 plasmid [GmR] | This study |
| pSL3361 | Ptrc1O-sps-spp (Synechocystis 6803), RSF1010 plasmid [GmR] | This study |
| Synechococcus 2973 strains | ||
| 2973-cscB | NS3::PlacUV5-cscB (created using pSL3037) | This study |
| 2973-cscB-spp | 2973-cscB strain with plasmid pSL3360 | This study |
| 2973-cscB-sps-spp | 2973-cscB strain with plasmid pSL3361 | This study |