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. 2020 Jan 15;10:343. doi: 10.1038/s41598-019-57018-1

Figure 3.

Figure 3

Exo-1537S decrease viability of MDA-MB-231 cells. (A) Diagram of the different incubation periods of MDA-MB-231 cells with exosomes. MDA-MB-231 cells were incubated alone or in the presence of Exo-WT, Exo-C or Exo-1537 S (10 μg) for 6 h (B), 24 h (C) or 48 h (D) and proliferation was measured by MTT after switching to fresh medium. (E) Exo-1537S exosomes (10 μg) were treated with RNaseA or proteinase K, sonicated (5 min) or left untreated, after which they were added to MDA-MB-231 cells. Viability was determined by MTT assay at 24, 48 and 72 h. (F) MDA-MB-231 cells were pre-incubated for 6, 24 or 48 h alone or in the presence of Exo-WT, Exo-C or Exo-1537S exosomes. After switching to exosome-free fresh media, cells were further cultured for 48 h and viability was determined by Tb exclusion assay. (G) MDA-MB-231 cells were pre-incubated for 24 h either alone or in the presence of Exo-WT, Exo-C or Exo-1537S exosomes, followed by 48 h of culture in fresh media. Viability was determined by PI exclusion and flow cytometry analysis. The data correspond to the average of 3 independent experiments in triplicate. Statistically significant differences are indicated compared to wild-type cells (**p < 0.01).