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. 2020 Jan 9;7:432. doi: 10.3389/fbioe.2019.00432

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Copyright © 2020 Huang, Ferlez, Young, Kerfeld, Kramer and Ducat.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Higher-order assembly of shell-cytochromes. (A) Titration of potassium cyanide against 200 μM R₄ shell-cytochrome (based on heme concentration) at pH 7.8 monitored by Dynamic Light Scattering. (B) Titration curve of (A), showing the mass-weighted average particle sizes in different concentration of KCN. (C) Particle sizes of 200 μM shell-cytochromes R₁, R₂ and R₅ with 3 mM KCN. (D) Representative TEM micrograph of spherical shells (often broken) formed by combining H₅ with H. ochraceum BMC-P and BMC-T in an in vitro assembly reaction, as described in Hagen A. R. et al. (2018).