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. 2020 Jan 9;10:1564. doi: 10.3389/fphys.2019.01564

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Copyright © 2020 Makkos, Szántai, Pálóczi, Pipis, Kiss, Poggi, Ferdinandy, Chatgilialoglu and Görbe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative images of membrane cholesterol content in neonatal cardiac myocytes after cholesterol supplementation (hiChol1, hiChol2, hiChol3) measured with Filipin staining (for cholesterol content) and propidium iodide staining (for total cell count). (B) Membrane cholesterol levels of neonatal cardiac myocytes evaluated (ImageJ software) after cultivation with different supplements (hiChol1, hiChol2, hiChol3). Data are expressed as mean ± SEM compared to normoxia vehicle control group (100%). ^∗p < 0.05 vs. normoxia vehicle (one-way ANOVA, Tukey’s post hoc), n = 20–23.