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. 2020 Jan 15;10:295. doi: 10.1038/s41598-019-57099-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental flow of the trial. Before submaximal exercise, a maximal voluntary contraction (MVC) test was performed. Subjects breathed a normoxic gas mixture for 3 min (rest1). Next, the inspiratory gas mixture was either maintained or switched to a hypoxic gas mixture for 10 min (rest2). After the rest1 and rest2, subjects started isometric intermittent exercise consisted of 5 s of static knee extension with 50% MVC force followed by 5 s of rest for 3 min. The recovery time after the exercise was 1 min. The next trial (initiated by changing the gas mixture) started with same flow after a 15-min interval.