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. 2020 Jan 15;10:326. doi: 10.1038/s41598-019-57223-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) A 2D gray scale image preprocessed from experiment 1 fluorescent image (b) Binary Hessian convexity map of (a), the convex pixels are marked as the white color. (c) U-Net probability map of (a), pixel is illustrated with a color indicating a probability of the pixel belonging to a blob. (d) Blob identification map joined from Hessian convexity map and U-Net probability map with 0.5 threshold.