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. 2020 Jan 15;10:407. doi: 10.1038/s41598-019-57301-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Example images (A) and statistics (B) of mitochondrial ROS levels after VSMCs were stretched with V0, V6, V25 or V50 and labeled with MitoSOX™ Red mitochondrial superoxide indicator. Approximately 50 cells per group were included in the statistics. The medians are statistically different between all conditions. (C) Means and SDs of total ATP normalized to unity under V25. All conditions are statistically significantly different.