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. 2020 Jan 15;10:383. doi: 10.1038/s41598-019-57184-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glu-C peptide mapping results of the N325-containing peptide YKCKVSNKALPAPIE for 40 °C IEC prepeak and main peak fractions. The fraction name is provided on the right side of each panel. (A) Extracted ion chromatography of YKCKVSNKALPAPIE and its deamidated peptide. (B) Mass spectrometry spectra corresponding to 23.3 minutes (top), 23.5 minutes (middle), and 24.2 minutes (bottom). m/z mass/charge ratio.