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. 2020 Jan 15;10:418. doi: 10.1038/s41598-019-57383-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Seed germination assay under salt and osmotic stress. The germination rate of WT and transgenic seeds was detected on a medium supplemented with (a) 200 mM NaCl and (b) 300 mM Sorbitol for 7 d. (c) Control plate without any stress treatment was maintained for the same period of time. (d) Graphical representation of percentage seed germination after 7 d NaCl and osmotic stress treatments. H1 and H2 represent high expression lines whereas Low represents low expression line seedlings. Values are means ± SE (n = 3), and each replicate comprises 150 seeds. Different letters indicate significant difference (P ≤ 0.05) with each other.