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. 2020 Jan 15;10:418. doi: 10.1038/s41598-019-57383-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of osmotic stress on seedling growth. The 10 d old WT and transgenic seedlings were transferred to 200 mM (a) and 300 mM (b) Sorbitol medium for 15 days. (c) Seedlings not subjected to stress. (d) Phenotypic difference in the growth of seedlings after 15 d of treatment. (e) Graphical representation of root length measurements. Values are means ± SE (n = 3), and each replicate comprises 15 seedlings. (f) Measurement of CAT activity and H₂O₂ content after 10 d of stress treatment. Values are means ± SE (n = 3), and each replicate comprises minimum 10 seedlings. Different letters indicate significant difference (P ≤ 0.05) between each other. H1 and H2 represent high expression lines whereas Low represents low expression line seedlings.