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. 2020 Jan 15;10:418. doi: 10.1038/s41598-019-57383-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Leaf disc assay. (a) Stress tolerance exhibited by the leaf discs of WT and transgenic lines at various concentrations of NaCl (100, 200 and 300 mM) and (b) sorbitol 300, 400 and 500 mM treatments. Photographs were taken post 3 d NaCl and 5 d Sorbitol treatments. (c) Total chlorophyll and TBARS were measured in WT and transgenic leaf discs after 2 d NaCl treatment and after 4 d Sorbitol treatment. Values are means ± SE (n = 3), and each replicate comprises minimum 15 leaf discs. Different letters indicate significant difference (P ≤ 0.05) between each other. H1 and H2 represent high expression lines whereas Low represents low expression line seedlings.