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. 2020 Jan 15;10:418. doi: 10.1038/s41598-019-57383-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ROS detection in leaf epidermal guard cells using Confocal Microscopy. Fluorescence levels in WT and high expression line plants (H1 and H2) were observed after (a) control and (b) 100 mM NaCl treatments by staining with H₂DCFDA. WT samples showed higher ROS accumulation during NaCl stress. Bright field images were also displayed. (c) Quantification of ROS production by using Image-J software. Values are means ± SE (n = 3), and each replicate comprises 100 stomata. Different letters indicate significant difference (P ≤ 0.05) between each other.