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α-Diversity metrics are altered by a high-fat, high-calorie diet (HFCD) and metformin. Microbial diversity was assessed by Chao1 index (richness), Faith’s phylogenetic diversity, and Shannon index (evenness/richness) across different regions of the gastrointestinal tract. *Significance between control diet (CD) and HFCD; #significance between CD and HFCD + metformin.
