Fig. 1.

Role of CXC chemokine receptor 2 (CXCR2) on liver injury after bile duct ligation (BDL). A: liver injury was measured by serum level of alanine aminotransferase (ALT). B: liver histology was assessed by staining with hematoxylin and eosin. Normal hepatic architecture was observed in sham-operated wild-type (WT) mice and CXCR2−/− [knockout (KO)] mice. After 3 and 14 days, livers from wild-type mice had large areas of necrosis, whereas livers from CXCR2−/− mice showed less evidence of necrosis. Original magnification: ×50. Liver injury was quantitated by percentage of necrosis area histologically. BDL caused an increase in ALT and necrosis area; however, CXCR2−/− mice showed significantly lower ALT and necrosis area than wild-type mice at 3 and 14 days after BDL. C: mRNA expression of inflammatory cytokines in the liver after BDL. There was no significant difference in the TNF-α, IL-1β, IL-6, and IL-12 expression after BDL between wild-type mice and CXCR2−/−mice except TNF-α expression at 14 days. Data are means ± SE with n = 3–9 per group. *P < 0.05, compared with sham. #P < 0.05, compared with wild type.