Fig. 3.

Somatostatin (SS) receptor SSTr5 mRNA is more expressed in GLP-1 positive cells than SSTr2 mRNA, and none of them show high mRNA levels in the SS-positive cells. Dual in situ hybridization and immunohistochemical staining of SSTr2 and SSTr5 mRNA in small intestinal D- and L-cells. A: representative images of immunohistochemically stained GLP-1 cells (green) and in situ hybridization stained SSTr2 or SSTr5 mRNA (red dots) and a merge image (third picture) performed on mouse jejunum or proximal ileum sections. B: the same as above, however, with SS immunolabeled cells. Each red dot represents a single stained mRNA transcript. Dashed line outlines SS cell borders. Nuclei were visualized with DAPI counterstaining (blue). Ab, antibody; Pb, probe; bar = 5 µm. Male mice, n = 4.