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Comparison of unconjugated (free) androgen levels in intestine and extraintestinal tissues of conventionally raised (CONV-R) and germ-free (GF) male mice: dihydrotestosterone (DHT; A), testosterone (T; B) and androstenedione (A-dione; C). Values below lower limit of quantification (LLOQ) are set to LLOQ. Values are shown as means ± SE; n = 10 per group. Significance: *P < 0.05; **P < 0.01; ***P < 0.0001 according to Student’s 2-tailed unpaired t test. Sem ves, seminal vesicles.
