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. 2019 Oct 8;317(6):E957–E972. doi: 10.1152/ajpendo.00109.2019

Fig. 2.

Fig. 2.

Exocyst subunit EXOC5 colocalizes with glucose transporter type 4 (GLUT4) in skeletal myoblasts under basal and insulin-stimulated conditions. A, C, and E: confocal images of untreated (top panels) and insulin-induced (bottom panels) L6-GLUT4myc rat skeletal myoblasts immunostained for exocyst subunits (using anti-EXOC1, EXOC5, or EXOC7 antibodies) and GLUT4myc [using anti-myc (A and C) or anti-GLUT4 (E) antibodies]. Nuclei were stained with DAPI (blue). Scale bars: 10 µm. B, D, and F: GLUT4myc and exocyst subunit colocalization expressed as average coefficients of correlation (Pearson’s). Data presented are representative of 3 independent preparations. Values are means ± SD in relative units (vs. untreated cells); n, no. of images analyzed. **P < 0.01, ***P < 0.005, and ****P < 0.001 compared with untreated control.