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. 2019 Oct 8;317(6):E957–E972. doi: 10.1152/ajpendo.00109.2019

Fig. 3.

Fig. 3.

Exocyst subunits show an increased association with glucose transporter type 4 (GLUT4) storage vesicles in response to insulin in myoblasts. Colocalization of exocyst subunits EXOC5 and EXOC7 with GLUT4 vesicles was demonstrated using the proximity ligation assay, where immunofluorescent signal (red dots) indicates the close proximity between the target proteins. Confocal microscopy images and quantification of a proximity ligation assay interrogating EXOC5 and GLUT4 proximity (A) and EXOC7 and GLUT4 association (B) in untreated and insulin-induced L6 GLUT4myc myoblasts are shown. Nuclei were stained with DAPI, and results were quantified by counting the number of red signals per nuclei, with a minimum of 5 samples (at least 100 cells total) counted for each assay. Scale bar = 10 µm. Values are means ± SD in relative units (vs. wild-type L6 GLUT4myc cells); n, no. of images analyzed. **P < 0.01. ***P < 0.005.