Fig. 4.
Diurnal change in sodium-chloride cotransporter (NCC) phosphorylation at Thr53 (T53) is abrogated by chronic RU-486 treatment. Male C57BL6 mice were treated for 5 days with RU486 slow-release pellets or vehicle and terminated in pairs every 6 h starting at Zeitgeber time (ZT) 0 (lights on, local time 7:00 AM). Summary data are presented for Western blots run in parallel (A), and Western blots are presented in B and C. R, reference pool of kidney homogenate loaded on all gels; 2×, 2× loading control to test linearity. Data are means ± SD and analyzed by two-way ANOVA with post hoc Sidak’s tests comparing data within the same genotype to the trough levels at ZT6, n = 5–6 biological replicates.