Fig. 5.

Diurnal variation in gene expression in mice treated with chronic RU486 (white triangles) or vehicle (black circles). Male C57BL6 mice were treated for 5 days with RU486 slow-release pellets or vehicle and terminated in pairs every 6 h starting at Zeitgeber time (ZT) 0 (lights on, local time 7:00 AM). Kidneys were taken for quantitative RT-PCR. Clock, circadian locomotor output cycles protein kaput; Arntl, aryl hydrocarbon receptor nuclear translocator-like protein 1; Cry1, cryptochrome 1; Cry2, cryptochrome 2; Per1, period circadian protein homolog 1; Per2, period circadian protein homolog 2; Nrld1, nuclear receptor subfamily 1 group D member 1; Nrld2, nuclear receptor subfamily 1 group D member 2; Tsc22d3 (Gilz), glucocorticoid-induced leucine zipper protein; Sgk1, serum- and glucocorticoid-induced kinase 1. Data are means ± SD and were analyzed by two-way ANOVA followed by post hoc Sidak’s tests, n = 5–6 biological replicates. See Table 1 for gene descriptions and protein names.