Fig. 6.

Double staining confirmed laminin-β₂ (LAMB2) expression in parietal epithelial cells (PECs) in mice with experimental focal segmental glomerulosclerosis (FSGS). A and B: double staining for nephrin (red) and LAMB2 (green). A and A³: a normal mouse glomerulus. The merged image shows staining for LAMB2 (green) in the glomerular basement membrane distribution, with nephrin staining (red) alongside. A¹ and A²: staining for nephrin (A¹) and LAMB2 (A²) showing their absence along Bowman’s capsule. B and B³: FSGS. The merged image shows de novo LAMB2 but not nephrin staining along Bowman’s capsule. B¹ and B²: nephrin (B¹) and LAMB2 (B²) staining. Higher magnification of the inset in B showed no staining for nephrin (B⁴) along Bowman’s capsule in areas staining for LAMB2 (white arrows) (B⁵). Their merged image is shown in B⁶. C and D: double staining for β-Gal (red) and LAMB2 (green). C and C³: a normal glomerulus. The merged image shows that there was no overlap of LAMB2 staining in the glomerular basement membrane distribution and β-Gal, which permanently labels PECs. C¹ and C²: β-Gal and LAMB2. D and D³: FSGS. The merged image shows de novo staining for LAMB2 in a Bowman’s capsule distribution (D¹ and D²). Higher power magnification of the inset D shows that β-Gal (yellow arrows) (D⁴) is lined by LAMB2 (white arrows) (D⁵), as easily seen in the merged image (D⁶). Original magnification: ×400. Scale bars = 20 μm.