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Cryptosporidium parvum infection decreased downregulated in adenoma (DRA) immunostaining in mouse enteroid-derived monolayers. Mouse ileal crypt-derived enteroids and enteroid-derived monolayers were prepared as described in experimental procedures. Monolayers treated with C. parvum from apical surface for 24 h were processed for immunofluorescence staining for DRA (green) and DAPI (blue).
