Fig. 2.

Mechanical overload. A: synergist ablation model. Wild-type (WT) and eukaryotic initiation factor 4E (eIF4E) knock-in (KI) mice (5-mo-old) were subjected to synergist ablation (SA) surgery or a sham operation. Muscles were collected 3, 7, and 14 days after surgery. Sham mice were collected across all time points. B: Western blot for phosphorylated (Ser²⁰⁹) and total eIF4E in response to mechanical overload. The closest molecular weight marker is indicated to show the approximate weight of the target protein. C: quantification of eIF4E phosphorylation. D: quantification of total eIF4E. ND, not detected; AU, arbitrary units. Values are means ± SE; n = 6 mice per group at each time point after surgery, as well as sham operation. *Statistical significance vs. sham.