Fig. 4.

Ribosomal DNA transcription and translational capacity changes during mechanical overload in wild-type (WT) and eukaryotic initiation factor 4E (eIF4E) knock-in (KI) mice subjected to synergist ablation (SA) surgery or a sham operation. A and B: changes in 47S pre-rRNA, as measured by 5.8S-ITS and ITS (n = 6 mice per group). C and D: translational capacity markers (n = 6–9 mice per group). C: total RNA per milligram of muscle tissue. D: rRNA per milligram of tissue. Inset: representative image of gel electrophoresis of rRNA depicting 28S and 18S rRNAs. AU, arbitrary units. Values are means ± SE. *Statistical significance vs. sham.