(A) Flow cytometry analysis of small intestine lamina propria (siLP) lymphocytes. Dead cells were excluded by a fixable viability dye, and the Lin−CD127+ ILCs were further gated for analyses in (B) and (C).
(B) Residual ILC (Lin−CD127+) population in the siLP of the Gata3fl/flVavCre mice were characterized by intracellular GATA-3 and RORγt staining.
(C) The NKp46+ ILC3 subset (RORγt+NKp46+) among the siLP ILCs (Lin−CD127+) was analyzed.
(D) The RORγt+NKp46− ILC3s shown in (C) were further characterized based on their CCR6 and T-bet expression.
(E) The percentage of LTi cells (CCR6+ ILC3s) among all IL-7R-expressing ILCs were compared (mean ± s.d.; n = 5–6; ***P < 0.001, Student’s t-test).
(F) The total CCR6+ LTi cell numbers in the siLP were compared (mean ± s.d.; n = 5–6; ***P < 0.001, Student’s t-test).
Data are representative of at least three independent experiments (A-F).
See also Figure S1.