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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: Immunity. 2019 Dec 24;52(1):83–95.e4. doi: 10.1016/j.immuni.2019.12.001

Figure 1. GATA3 is Required for the Development of All Non-LTi ILCs but Dispensable for the LTi Cell Generation.

Figure 1.

(A) Flow cytometry analysis of small intestine lamina propria (siLP) lymphocytes. Dead cells were excluded by a fixable viability dye, and the Lin−CD127+ ILCs were further gated for analyses in (B) and (C).

(B) Residual ILC (Lin−CD127+) population in the siLP of the Gata3fl/flVavCre mice were characterized by intracellular GATA-3 and RORγt staining.

(C) The NKp46+ ILC3 subset (RORγt+NKp46+) among the siLP ILCs (Lin−CD127+) was analyzed.

(D) The RORγt+NKp46− ILC3s shown in (C) were further characterized based on their CCR6 and T-bet expression.

(E) The percentage of LTi cells (CCR6+ ILC3s) among all IL-7R-expressing ILCs were compared (mean ± s.d.; n = 5–6; ***P < 0.001, Student’s t-test).

(F) The total CCR6+ LTi cell numbers in the siLP were compared (mean ± s.d.; n = 5–6; ***P < 0.001, Student’s t-test).

Data are representative of at least three independent experiments (A-F).

See also Figure S1.