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. Author manuscript; available in PMC: 2021 Jan 14.
Published in final edited form as: Immunity. 2019 Dec 24;52(1):83–95.e4. doi: 10.1016/j.immuni.2019.12.001

Figure 2. GATA3 is Indispensable for the Generation of the PLZF+ Non-LTi ILC Progenitors.

Figure 2.

(A) Flow cytometry analysis of common helper-like innate lymphoid progenitors (ChILPs) in the bone marrow (BM). The plots were gated on the live Lin−CD127+Flt-3−α4β7+ population. T1/ST2-Id2-YFPhigh cells were further analyzed for the expression of other molecules as shown in (B).

(B) The expression of representative molecules by the ChILP (live Lin−CD127+Flt-3−α4β7+T1/ST2−Id2-YFP+) cells in the presence (black line) or absence (red line) of Gata3 was analyzed by flow cytometry.

(C) The total numbers of αLP cells (live Lin−CD127+Flt-3−α4β7+T1/ST2-) were counted and plotted (mean ± s.d.; n = 6–7; ***P < 0.001, Student’s t-test). (D) The PLZF+ non-LTi ILC progenitors were analyzed by flow cytometry through gating on the live Lin−CD127+Flt-3−T1/ST2− cell population.

(E) The total numbers of ChILPs (live Lin−CD127+Flt-3−α4β7+T1/ST2−Id2-YFP+), ILCPs (live Lin−CD127+Flt-3−α4β7+T1/ST2−Id2-YFP+PLZF+) and ILC2Ps (live Lin−CD127+Flt-3−α4β7+T1/ST2+) were counted and plotted (mean ± s.d.; n = 3; **P < 0.01, ***P < 0.001, Student’s t-test).

Data are representative of three independent experiments (A-E).

See also Figure S2.