Skip to main content
. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Matrix Biol. 2019 Jul 16;85-86:80–93. doi: 10.1016/j.matbio.2019.07.006

Figure 3:

Figure 3:

Increasing stiffness upregulated Mena expression in spheroids, which was necessary for invasion in stiff gels but not soft ones. A) Immunofluorescent staining of spheroids demonstrated Mena upregulation began early and persisted throughout the duration of the experiment. Scale bar=150 μm. Expression of B.) MENA and C.) MENA-INV was upregulated at 12 and 24 hours in 12 kPa scaffolds compared to the 2 kPa condition. * denotes p<0.05 relative to low stiffness at same time point, n=3 pools of >50 embedded spheroids. D.) siRNA knockdown of MENA did not affect invasion in soft gels but greatly reduced it in stiffer conditions. * denotes p<0.05 relative to siScr control, n=7–12 spheroids per condition.