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Effect of RTD-1 on gene expression by IL-1β-stimulated RA-FLS cells. RA-FLS cells were stimulated with IL-1β ± RTD-1 for 6 h, and the RNA was used to analyze the expression of human genes by qRT-PCR and normalized to ACTB gene expression. Data show means ± SD (t test, two tailed: *P < 0.05, **P < 0.01; comparison of IL-1β + RTD-1 with IL-1β treatment). With the exception of GAPDH, addition of RTD-1 significantly suppressed expression of each gene.
