Fig. 2.
A: schematic of the ORFeusLSL transgene: 1) the floxed allele of the ORFeusLSL transgene, 2) the excised allele of the ORFeusLSL transgene following Cre-mediated excision of the floxed β-geo-stop cassette (LSL), and 3) putative insertion upon retrotransposition. B: long interspersed nuclear element-1 (LINE-1) mRNA in lungs of ORFeusLSL mice treated with benzo(a)pyrene (BaP) alone or in combination with CRE recombinase. LINE-1 mRNA was measured by RT-qPCR using primers specific for ORFeusLSL. A (upright), polyadenylation signal; gfp, green fluorescent protein; LTR, long terminal repeat; ORF, open reading frame; RQ, relative quantification; n = 3 animals examined. Different letters denote differences between control, BaP-treated, and CRE-BaP-treated groups at P ≤ 0.05.