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. 2019 Nov 1;317(6):H1231–H1242. doi: 10.1152/ajpheart.00237.2019

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Fig. 6. — cAMP-dependent protein kinase (PKA) proteins are specifically enriched in subsarcolemmal mitochondria (SSM), and type I PKA regulatory α-subunit (RIα) expression is decreased in SSM with ischemia-reperfusion (I/R) injury. A: immunoblot analysis of PKA-associated proteins from untreated adult mouse heart ventricular tissue fractionated by differential centrifugation to separate mitochondrial subpopulations. AKAP1, A-kinase anchoring protein 1; COXVI, cytochrome-c oxidase; WCL, whole cell lysate; Pellet, nuclear/cytoskeleton pellet; Cyto, cytosol. B: immunoblot analysis of PKA-associated proteins within SSM subjected to density gradient ultracentrifugation. Mem, purified membrane; pSSM, purified SSM. C: immunoblot analysis of RΙα, catalytic subunit of PKA (PKAc), and COXIV from fractionated adult mouse heart ventricular tissue, comparing hearts that received either control perfusion or I/R injury. D: quantification of RIα expression from data in C, expressing changes of SSM-localized protein either with or without I/R injury stimulus (n = 3 biological replicates). Data are presented as means ± SD. *P < 0.05, compared on control condition.