Fig. 8.
Expression of BDNF, CRH, and adrenergic receptor mRNA in the PVN, and TH and DβH mRNA in the NTS of rats previously injected with AAV2-GFP (n = 6) or AAV2-BDNFmyc (n = 6) in the PVN. A: significant increases in BDNF and CRH mRNA expressions verify successful vector mediated BDNF transduction and activation of BDNF-mediated signaling mechanisms. B: expressions of α1a-, α1b-, and α2a- and β1- and β2-adrenergic receptor mRNAs in GFP and BDNF rats. C: expressions of TH and DβH mRNAs in the NTS of AAV2-GFP (n = 7) and AAV2-BDNFmyc (n = 7) rats. PVN, paraventricular nucleus; NTS, nucleus of the solitary tract; GFP, green fluorescent protein; BDNF, brain-derived neurotrophic factor; BDNFmyc, myc epitope-tagged BDNF; TH, tyrosine hydroxylase; DBH, dopamine β-hydroxylase; AAV2, adeno-associated viral vector 2; CRH, corticotropin-releasing hormone. Results are expressed as 2−ΔΔCT (where CT is threshold cycle) normalized to the control group and presented as means ± SE. **P < 0.01 GFP vs. BDNF (unpaired t test).