Vasculogenesis-Disabled Phenotype of KDR−/– Fetus and Its Compensation by Blastocyst Complementation
(A) A PDX1/KDR dual KO (DKO) fetus with vasculogenesis-disabled phenotype (left) and a chimeric fetus with normalized trait (right) obtained after blastocyst complementation.
(B) Left: a PDX1/KDR-KO fetus showing retarded development without blood flow. Center: the appearance of a chimeric fetus showing systemic chimerism with dense distribution of the exogenous huKO-expressing cells. Right: morphology and fluorescence intensity of a normal fetus derived from the huKO transgenic cell. Scale bars, 1 mm.
See also Figure S2.