Figure 6.

Improved Endocrine Differentiation Following Reduction of CDX2 Expression in PPs

(A) Flow cytometry-based quantifications of stage 5 endocrine progenitor differentiation of protocol B and the two modified conditions (condition 5 and 3). Scatterplots show percentage of NEUROD1 and NKX2.2-positive cells as means ± SEM, n = 5 independent experiments (three with SB AD3.1 hiPSC, one with SB AD3.4 hiPSC, and one with SA121 hESC).

(B) Immunofluorescence microscopy images of stage 6 cells stained with NKX6.1 and C-peptide antibodies. DAPI is used to visualize the nuclei of all cells. Scale bar, 100 μm.

(C) Representative pseudo color dot plots of stage 6 beta-like cells stained for C-peptide and NKX6.1. Numbers mark the percentage of cells in each quadrant.

(D) Quantification of C-peptide/NKX6.1 double positive by flow cytometry as shown in (C) (conditions 5 and 3). Scatterplot shows percentage of C-peptide/NKX6.1 double-positive cells as means ± SEM, n = 6 independent experiments (four with SB AD3.1 hiPSC, two with SA121 hESC).

(A and D) One-way ANOVA with Tukey test for multiple comparisons, ^∗∗∗p < 0.001, different from the two other groups.