Figure 11.

Biochemical Characterization of DNAzymes and Ribozymes

(A) The secondary (left) and crystal structure (right) of the RNA-cleaving DNAzyme, 8-17, revealing a V-shaped overall folding (PDB: 5XM8).

(B) A proposed mechanism of 8-17 catalysis including a Pb²⁺-bound water molecule as a general acid and a conserved guanine (G13) as a general base. Reprinted with permission from Liu et al. (2017a). Copyright © 2017 Springer Nature.

(C) Phosphorothioate modification on the scissile phosphate.

(D) Na⁺-induced folding enhances the fluorescence of a 2AP-modification at the cleavage site of the Ce13d DNAzyme.