Figure 8.

Representative RNA-Cleaving DNAzymes Selected with Non-metal Targets

(A) The L-histidine DNAzyme.

(B) An in vitro selection strategy to identify DNAzymes using the crude extracellular mixture from cells. The two nucleotides near the cleavage site are labeled with a fluorophore and a quencher, respectively.

(C) The sequence of the RFD-CD1 DNAzyme and its cleavage activity with respect to various species of bacteria (top) and various strains of C. difficile (bottom). Reprinted with permission from Shen et al. (2016). Copyright © 2015 Wiley-VCH.

(D) The VAE-2 DNAzyme responsive to the crude extracellular mixture of Vibrio anguillarum.