Figure 2.

G6P accumulation can increase the expression of Txnip. (A) Changes in expression of Txnip after treating with 2-DG 0–25 mM; (B) The expression of Txnip and its transcription factor MondoA in 2 h time scale treated by 200 μM 2-DG; (C,G) The protein and mRNA expression levels of Txnip after knockdown of two catalytic enzymes (PGI and G6PD) (T-test, *P < 0.05; **P < 0.01; ***P < 0.001); (D,E) The protein levels of Txnip after knockdown of two catalytic enzymes (PGI and G6PD); (F) The quantification amount of G6PD, PGI, and Txnip expression in HeLa cells after knockdown PGI and G6PD; (H) Separation efficiency of the nuclear isolation of cultured Bcap37 cells; (I) MondoA binding efficiency in the EMSA experiment increased with the different concentrations of G6PNa₂ added to the reaction system.