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. 2020 Jan 9;6:147. doi: 10.3389/fmolb.2019.00147

Figure 4.

Figure 4

The effects of several key point mutations on MondoA on the Txnip promoter. (A,B) The cells were transfected with mutant plasmids S282G, T284A, S287A, DEL282-287 and 139-141 mut, DEL39-141, and the wild-type plasmid for overexpression testing (Flag tag). (A) Vector, WT, S282A, T284A, S287A, and DEL282-287, (B) Vector, WT, S287A, 139-141GAA, and DEL139-141; (C,D) Detection of MondoA activity by the dual luciferase reporter gene assay under 25 mM glucose for 4 h (T-test, *P < 0.05; **P < 0.01; ***P < 0.001). (C) WT, S282A, T284A, S287A, and DEL282-287; (D) WT, S287A, 139-141GAA, DEL139-141, and Flag-vector.