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. 2019 Dec 23;42(6):371–380. doi: 10.1016/j.bj.2019.05.005

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© 2019 Chang Gung University. Publishing services by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 5 — Potential for chondrogenic differentiation of immortalized hTERT-ACL-MSCs. After immortalization hTERT ACL cells of young (hTERT ACL_young) and old (hTERT ACL_old) donors were differentiated towards the chondrogenic lineage for 21 days. Chondrogenic differentiation (induced) was evaluated compared to controls (non-induced) using (A) staining for proteoglycans using alcian blue, (B) staining with haematoxylin and eosin (H&E) to illustrate cell nuclei, and (C) immunohistochemistry for collagen (COL) 2 with bars indicating 200 μm. (D) Chondrogenic marker gene expression for the chondrogenic marker genes aggrecan (ACAN), biglycan (BCN), COL2 (COL2A1), COL10 (COL10A1), cartilage oligomeric matrix protein (COMP), and fibromodulin (FMOD) was detected by RT-PCR analyses, with EEF1A1 serving as internal control. Co: Control; Ind: Induced.