Table 1.
Primers and PCR conditions.
| Gene | Forward and reverse primer | Product length | Annealing temperature | MgCl2 Concentration | Accession ID (NCBI) |
|---|---|---|---|---|---|
| Adipogenic markers | |||||
| LPL | F: 5′-CTGCAAATGAGACACTTTCTC-3' R: 5′-CATGTCCTGGCAGTCTCTGA-3′ |
276 bp | 51 °C | 1.0 g/l | NM_000237 |
| PPARG | F: 5′-GCTGTTATGGGTGAAACTCTG-3' R: 5′-TAAGGTGGAGATGCAGGCTC-3′ |
350 bp | 51 °C | 1.0 g/l | NM_005037, NM_015869, NM_138711, NM_138712 |
| Osteogenic marker | |||||
| ALPL | F: 5′-TGGAGCTTCAGAAGCTCAACACCA-3' R: 5′-ATCTCGTTGTCTGAGTACCAGTCC-3′ |
454 bp | 51 °C | 1.0 g/l | NM_000478, NM_001127501, NM_001177520 |
| Chondrogenic markers | |||||
| ACAN | F: 5′-GCCTTGAGCAGTTCACCTTC-3' R: 5′-CTCTTCTACGGGGACAGCAG-3′ |
392 bp | 54 °C | 1.0 g/l | NM_001135, NM_013227 |
| BGN | F: 5′-AGTAGAGGCAGCTGCAGCCAC-3' R: 5′-GTGTCTGGACAGGTGCGGCAGC-3′ |
424 bp | 53 °C | 1.0 g/l | NM_001711 |
| COL2A1 | F: 5′-GGTATTGCTGGCTTCAAAGG-3' R: 5′-AGGACGACCATCTTCACCAG-3′ |
387 bp | 58 °C | 1.0 g/l | NM_001844, NM_033150 |
| COMP | F: 5′-CAGGACGACTTTGATGCAGA-3' R: 5′-AGGCTGGAGCTGTCCTGGTA-3′ |
312 bp | 54 °C | 1.0 g/l | NM_000095 |
| FMOD | F: 5′-ACCAGTGATAAGGTGGGCAG-3' R: 5′-AAGTAGCTATCGGGGACGGT-3′ |
368 bp | 54 °C | 1.0 g/l | NM_002023 |
| Hypertrophic marker | |||||
| COL10A1 | F: 5′-GGTATTGCTGGCTTCAAAGG-3' R: 5′-AGGACGACCATCTTCACCAG-3′ |
468 bp | 54 °C | 1.0 g/l | NM_000493 |
| Housekeeping gene | |||||
| EEF1A1 | F: 5′-AGGTGATTATCCTGAACCATCC-3' R: 5′-AAAGGTGGATAGTCTGAGAAGC-3′ |
235 bp | 54 °C | 1.0 g/l | NM_001402 |
Illustrated are the forward and reverse primers to amplify specific gene products along with their associated product lengths (lAmplicon), the annealing temperature (TAnneal), the concentrations of MgCl2 used in the reaction mixture (cMgCl), and the accession ID at the Gene database of the National Center for Biotechnology Information (NCBI).