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. 2018 Aug 1;11(8):3943–3951.

Figure 5.

Figure 5

HK2 was a target gene of miR-493-5p in cervical cancer cells. A. HK2 was predicted as a potential target gene of miR-493-5p. The prediction result was from Targetscan. B. A luciferase assay was used to analyze the luciferase activity of HK2 3’UTR in HeLa cells. The cells were co-transfected with miR-493-5p mimics and HK2 3’UTR and then the dual luciferase system was used to measure the luciferase activity. C. MiR-493-5p down-regulated HK2 mRNA levels in cells. The cells were transfected with miR-493-5p mimics and then HK2 mRNA was analyzed by real time RT-PCR. D. MiR-493-5p down-regulated HK2 protein levels in cells. The cells were transfected with miR-493-5p mimics and then HK2 was immunoblotted by Western blotting. *P<0.05, **P<0.01.