Figure 3.

Cyclophosphamide down-regulated transcription activities of cytochrome P450 family genes involved in ovarian steroid metabolism in a targeted fashion in mOGCs. A. Clustered index results of differentially expressed genes involved in ovarian steroid genesis. KEGG pathway analysis identified a group of genes belonging to the functional group of ovarian steroid genesis, and its transcription activity was significantly lower in POF mOGCs than in the control group. Of the 122 genes, we screened 20 genes with log2 [POF/PBS] < -3.0. B. Prediction results using the String online tool for the differential transcripts suggest that there is interaction between the 10 proteins of the ovarian steroid genesis functional group. C. Co-IP western blot results show that the expression levels of Fdx1, Cyp2u1, Cyp12a1, and Cyp11a1 in the POF group are significantly lower than those in the PBS group, *P < 0.05 vs. PBS group, n = 3.