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Thrombospondin (TSP)-1 and TSP-2 expression level assays in the aud cortex using immunohistochemistry and histogram analysis. A. Immunohistochemistry assay of TSP-1 and TSP-2 in aud cortex; B. Histogram analysis of TSP-1 expression levels in the aud cortex; C. Histogram analysis of TSP-2 expression levels in the aud cortex. The images indicate that TSP-1/TSP-2 expression levels in the aud cortex significantly increased with increasing reperfusion times (**: P<0.01, compared to sham).
